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KMID : 0861020170320020017
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Korea Journal of Herbology
2017 Volume.32 No. 2 p.17 ~ p.24
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Anti-inflammatory Effects of Ethanol Extract of Various Korean Compositae Herbs in LPS-induced RAW 264.7 Macrophages
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Seo Min-Gyu
Kang Yun-Mi
Chung Kyung-Sook
Cheon Se-Yun
Park Jong-Hyuk
Lee Young-Cheol
An Hyo-Jin
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Abstract
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Objective : This study was designed to evaluate candidate materials as anti-inflammation agent from extracts of various Korean Compositae herbs in Hwaak mountain. Among Korea medicinal herbs, Ainsliaea acerifolia (AA) belongs to the Compositae family, has been used for the treatment of rheumatic arthritis. However, AA has not been previously reported to have an anti-inflammatory effect. Therefore, we investigated the anti-inflammatory effects of AA and its underlying molecular mechanisms in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages.
Methods : Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in RAW 264.7 macrophages. Nitric oxide (NO) was measured with Griess reagent and pro-inflammatory cytokines were detected by enzyme immunoassay (EIA) kits in LPS-stimulated RAW 264.7 macrophages. Protein expressions of inducible nitric oxide synthase, and cyclooxygenase-2 (COX-2) and p65 subunit of nuclear factor-κB (NF-κB) were determined by Western blot analysis.
Results : Among 8 extracts of Korean Compositae herbs tested, AA showed the inhibition of NO production without cytotoxicity. Consistent with the observation, AA reduced the expression levels of iNOS and COX-2 proteins in LPSsimulated RAW 264.7 macrophages in dose-dependent manner. In addition, AA inhibited the productions of TNF-α and IL-6 in LPS-simulated RAW 264.7 macrophages. However, AA did not inhibit activation of p65 NF-κB in LPSsimulated RAW 264.7 macrophages.
Conclusion : These results suggest that down-regulation of iNOS, COX-2 protein expression and TNF-α and IL-6 production by AA are responsible for its anti-inflammatory effects.
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KEYWORD
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Ainsliaea acerifolia (AA), anti-inflammation, lipopolysaccharide (LPS), nitric oxide (NO), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2)
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